Journal: Nature Communications

Article Title: Prussian blue nanoparticles targeting multiple PANoptosome-mediated PANoptosis for myocardial ischemia-reperfusion injury therapy

doi: 10.1038/s41467-026-70012-2

Figure Lengend Snippet: A Schematic of ischemic zone (IZ), remote zone (RZ), and border zone (BZ) in human acute myocardial infarction. The heart element in the image was sourced from BioRender (Created in BioRender. xu, L. (2026) https://BioRender.com/9kka4p3 ). B UMAP visualization of cell distribution (left) and annotated cell types (right) in the control ( n = 4) versus IZ ( n = 11) groups. C Cardiomyocyte subpopulation quantification (left) and UMAP-based clustering (right) in the control and IZ groups. D , E UMAP projection ( D ) and box plots ( E ) showing PANoptosis activation across cardiac cell types. (The exact n in E = [left to right] 20, 84 cells; 17326, 3506 cells; 6231, 6969 cells; 9468, 12187 cells; 3386, 7142 cells; 3039, 2286 cells; 583, 254 cells; 531, 1138 cells). The minima, maxima, mean, median, bounds, whiskers, and percentile information were provided in the Source Data file. Exact P- values from left to right: 2.39E-265, 1.55E-300, 1.05E-35, 4.09E-05, 2.21E-06, 0.0002, 1.9E-11. F Violin plots comparing PANoptosis-related gene expression ( AIM2, ZBP1, RIPK1, Pyrin, NLRP12, NLRP3, MLKL, GSDMD, RIPK3, caspase 3, caspase 1 , and caspase 8 ) in total cells. ( n = 40706 cells [control], 33688 cells [IZ]). The minima, maxima, mean, median, bounds, whiskers, and percentile information were provided in the Source Data file. Exact P- values from left to right ( AIM2 to caspase 8 ): 0.2752, 0.0002, 0, 0.0854, 6.67E-09, 7.19E-82, 3.59E-37, 0.0709, 0.0269, 2.29E-280, 2.45E-30, 4.07E-09. G, H Cardiomyocyte-specific UMAP ( G ) and quantitative PANoptosis levels ( H ) of the control and IZ groups. (The exact n in H = [left to right] 6904 cells, 0; 5281 cells, 0; 1191, 3346 cells; 3192, 80 cells; 758, 80 cells). The minima, maxima, mean, median, bounds, whiskers, and percentile information were provided in the Source Data file. Exact P- values from left to right: 4.01E-05, 0.0021. I Proportional representation of cardiomyocyte subpopulations. J , K Heatmap depicting activation patterns of apoptosis/PANoptosis/pyroptosis/necroptosis ( J ) and PANoptosis-related genes ( K ) across cardiomyocyte subtypes. Significance: wilcox.test, a two-sided test; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001; ns : no significant difference ( P > 0.05). Source data are provided as a Source Data file.

Article Snippet: Necrostatin-1 (HY-15760), Disulfiram (HY-B0240), Z-VAD-FMK (HY-16658B), ZBP1 (HY- P83326 ), phospho-RIPK1 (HY- P81539 ), phospho-MLKL (HY- P80468 ), cleaved caspase 1 (HY- P80622 ), cell counting kit-8 (CCK-8) assay kit (HY-K030), and cleaved caspase 8 (HY- P80624 ) were obtained from MedChemExpress, USA.

Techniques: Control, Activation Assay, Gene Expression

Journal: Nature Communications

Article Title: Prussian blue nanoparticles targeting multiple PANoptosome-mediated PANoptosis for myocardial ischemia-reperfusion injury therapy

doi: 10.1038/s41467-026-70012-2

Figure Lengend Snippet: A PCA of RNA-seq data (Sham: orange; MIRI: green; PB@PM: blue; n = 5 biologically independent replicates). B Volcano plot of differentially expressed genes (DEGs; PB@PM vs. MIRI: red/blue = up/downregulated). The Wald test of DESeq2 (DESeq(dds) default test = “Wald”) was used, which is a two-sided test. Multiple comparisons correction: the FDR (False Discovery Rate) correction of the BH (Benjamini-Hochberg) method was computed to obtain P adjustment ( P .adj.). C Venn diagram of shared DEGs between Sham-MIRI and PB@PM-MIRI comparisons. D GSVA of pathway enrichment across groups. E KEGG pathway enrichment of downregulated genes in PB@PM vs. MIRI. A one-sided hypergeometric test was used. Multiple comparisons correction: the FDR correction for the BH method was calculated to obtain P .adj. F , G GSEA networks ( F ) and protein interaction networks ( G ) for pyroptosis, apoptosis, and necroptosis. H GSEA plots showing marked downward trends of pyroptosis, apoptosis, and necroptosis in the PB@PM group compared to the MIRI group. A permutation test was used, based on weighted Kolmogorov-Smirnov-like statistics. It is a two-sided test (tests whether the enrichment scores are significantly deviated from zero, which can be positive or negative, corresponding to up- or down-regulated enrichment, respectively). Multiple comparisons correction: The FDR correction for the BH method was calculated to obtain P .adj. I Pearson correlation between pyroptosis, apoptosis, and necroptosis in the Sham (C), MIRI (I), and PB@PM (T) groups. Using psych R package, method = “spearman” function, t-test, two-sided test, and no correction for multiple comparisons. J Western blot of PANoptosis-related proteins (AIM2, ZBP1, Pyrin, ASC, FADD, Bax, Bcl-2, phosphorylated/total RIPK1/RIPK3/MLKL, cleaved/total caspase-1/3/8 and GSDMD) in ischemic myocardium (day 3; n = 5 biologically independent replicates). Source data are provided as a Source Data file.

Article Snippet: Necrostatin-1 (HY-15760), Disulfiram (HY-B0240), Z-VAD-FMK (HY-16658B), ZBP1 (HY- P83326 ), phospho-RIPK1 (HY- P81539 ), phospho-MLKL (HY- P80468 ), cleaved caspase 1 (HY- P80622 ), cell counting kit-8 (CCK-8) assay kit (HY-K030), and cleaved caspase 8 (HY- P80624 ) were obtained from MedChemExpress, USA.

Techniques: RNA Sequencing, Western Blot

Journal: Molecules

Article Title: Suppressive Effect of Two Cucurbitane-Type Triterpenoids from Momordica charantia on Cutibacterium acnes -Induced Inflammatory Responses in Human THP-1 Monocytic Cell and Mouse Models

doi: 10.3390/molecules26030579

Figure Lengend Snippet: Antibodies used for Western blotting (WB) and flow cytometry (Flow).

Article Snippet: cleaved caspase-1 , Polyclonal , GeneTex (GTX134551) , WB.

Techniques: Western Blot, Flow Cytometry